A Fiber Alginate Co-culture Platform for the Differentiation of mESC and Modeling of the Neural Tube

Alginate hydrogels are a commonly used substrate for in vitro 3D cell culture. These naturally derived biomaterials highly tunable, biocompatible, and can be designed to mimic the elastic modulus of adult brain at 1% w/v solution. Recent studies show that molecular weight alginate affect viability differentiation. The relationship between weight, viscosity ratio G:M monomers is complex, balance these factors must carefully considered when deciding on suitable hydrogel stem research. This study investigates formation embryoid bodies (EB) from mouse embryonic cells, using low (LMW) high (HMW) alginates. cells differentiated retinoic acid-based protocol, resulting aggregates sectioned stained presence three germ layers (endoderm, mesoderm, ectoderm). results highlight within LMW HMW true EBs, as demonstrated by positive staining markers layers. Using tubular scaffolds, formed with an adapted gradient maker we also propose novel platform patterned differentiation mESCs, based gradients acid produced situ lateral motor column (LMC) neurons. end product our will great interest it further developed into powerful model neural tube development.